Cytogenetic pre natal analysis is the early detection of genetic diseases in a fetus or embryo before it is born. The aim is to detect birth defects such as neural tube defects, Down syndrome, chromosome abnormalities, genetic diseases and other conditions. It can also be used to determine the sex of the unborn baby. Common procedures used in pre natal cytogenetic diagnosis include amniocentesis and chorionic villisampling and long term culture of derived cells for a subsequent karyotyping.
Amniomed®Plus is a medium specifi cally developed for primary culture of amniotic fluid cells for foetal karyotyping. This medium promotes optimal in vitro adhesion and growth of amniocytes, reducing the time required for prenatal cytogenetic diagnosis, moreover it is optimised for both in situ and flask culture. Due to its special formulation, it is also suitable for culturing of chorionic villi, foetal tissues and skin fibroblasts. Amniomed®Plus is a complete medium (no additions required), sterile and readyto-use. Amniomed®Plus is supplied as frozen liquid, and as such can be stored at –20 ± 2°C for two years.
Comparative results against two leading competitor
Incubation time: all culture were harvested 7, 8 or 9 days after the initial plating.
Colony number: when compared on the same culture day and stained with Quinacrine, the Amniomed®Plus performance was equal to, if not better than, the competitor products.
Colony type: when compared on the same culture day and stained with Quinacrine, again the performance of Amniomed®Plus was equal to, if not better than, the competitor products.
Colony size:when compared on the same culture day and stained with Quinacrine, again the difference in size was not found to be statistically signifi cant.